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The microscope was equipped with a pulsed diode laser (18 mW 80 MHz 640 nm emission PicoQuant) for excitation of the STED dye and with a pulsed infrared titanium: sapphire (Ti:Sa) tunable laser (1W 80 MHz 720–1000 nm Mai Tai Broadband; Spectra‐Physics) for depletion set at a wavelength of 750 nm.
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Atto647N (part of mCLING's structure) and Chromeo 494 were excited with two pulsed diode lasers (PDL 800‐D PicoQuant Berlin Germany) emitting at 640 nm and 531 nm respectively. The STED beam was generated with a Ti:Sapphire laser (MaiTai Spectra‐Physics Darmstadt Germany) tuned at 750 nm.
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